NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 23,418.1
Mean read quality 18.5
Median read length 10,626.0
Median read quality 24.3
Number of reads 786,114.0
Read length N50 50,928.0
STDEV read length 29,467.2
Total bases 18,409,271,988.0
Number, percentage and megabases of reads above quality cutoffs
>Q10 763497 (97.1%) 18047.5Mb
>Q15 716221 (91.1%) 17293.6Mb
>Q20 609514 (77.5%) 14804.5Mb
>Q25 330433 (42.0%) 7364.8Mb
>Q30 34823 (4.4%) 99.2Mb
Top 5 highest mean basecall quality scores and their read lengths
1 45.1 (312)
2 45.0 (293)
3 45.0 (330)
4 44.4 (611)
5 44.3 (469)
Top 5 longest reads and their mean basecall quality score
1 471387 (23.1)
2 417050 (22.0)
3 404965 (25.6)
4 394461 (22.0)
5 392376 (16.3)

Summary statistics after filtering

General summary
Mean read length 23,419.1
Mean read quality 18.5
Median read length 10,627.0
Median read quality 24.3
Number of reads 786,081.0
Read length N50 50,928.0
STDEV read length 29,467.4
Total bases 18,409,271,788.0
Number, percentage and megabases of reads above quality cutoffs
>Q10 763464 (97.1%) 18047.5Mb
>Q15 716188 (91.1%) 17293.6Mb
>Q20 609481 (77.5%) 14804.5Mb
>Q25 330400 (42.0%) 7364.8Mb
>Q30 34790 (4.4%) 99.2Mb
Top 5 highest mean basecall quality scores and their read lengths
1 45.1 (312)
2 45.0 (293)
3 45.0 (330)
4 44.4 (611)
5 44.3 (469)
Top 5 longest reads and their mean basecall quality score
1 471387 (23.1)
2 417050 (22.0)
3 404965 (25.6)
4 394461 (22.0)
5 392376 (16.3)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality plot using dots after log transformation of read lengths